Active Ingredients: Hydroxyzine
The first section was white and second was black colored. The light illumination in the first section was kept bright and second was dim. Each mouse was placed separately in the center place of white box while facing door present in the partition.
The observation period of each animal was 10 min.
Parameters like time spent in light and dark area, percent time spent in light and dark area were analyzed from the recorded video by a trained person.
Euthanasia was performed 1 h after treatment and brain was isolated in ice cold 0. After recording weight of the brain, cerebral cortex, hippocampus, and remaining brain parts were removed and weighed separately.
Samples were homogenized in 2 ml of ice cold 0.
The supernatant was filtered through 0. The chromatographic separation was achieved on reversed-phase analytical column at room temperature, and the acquired data were processed using LC Solution software.
The mobile phase consists of sodium acetate 0. Mobile phase pH was adjusted using phosphoric acid pH-3. Flow rate of mobile phase was kept at 1.No hay comentarios Product of United.
Monoamines were detected at an excitation wavelength of 280 nm and an emission wavelength of 315 nm. Peaks were identified by comparing the retention time of sample and standard.
The concentration of each monoamine in the sample was analyzed according their area under curve using their straight line equation.
Statistical Analysis One-way ANOVA followed by Tukey's honest significant difference post-hoc test was used for the calculation of statistical significance. The GraphpadInStat for 32 bit Windows version 3.