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The dwell time for both levocetirizine and hydroxyzine monitoring was set at 200 ms.
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Samples were eluted with an isocratic mobile phase of acetonitrile and 10 mM ammonium formate pH 3. The entire flow was directed into the mass spectrometry source without splitting. The final concentration at 1.
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Structurally, levocetirizine gets to the diphenylpiperazine physician of compounds with selective H 1-antagonist hours.
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Preparation of clinical blood samples Blood samples were collected from six adult healthy volunteers who gave informed consent. Blood samples were taken using an indwelling cannula placed in a forearm vein and kept patent with normal saline.
Blood samples were collected in Na 2 EDTA blood collection tubes and centrifuged for 10 min at 3,200 g.
Method validation The analytical method was validated in terms of linearity, accuracy, precision, specificity, sensitivity, recovery and stability based on the US-FDA guidance for bioanalytical methods 11. Additionally, matrix effects and impact of hemolysis were also validated 12, 13.
It preparation using protein precipitation alone has like yet been applied for determination of levocetirizine in addition plasma.
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Specificity Specificity was evaluated by screening blank plasma from six different lots spiked with levocetirizine at the LLOQ.
The spiked samples were extracted and the presence or absence of interfering peaks at the same retention time of levocetirizine or hydroxyzine IS was examined.
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Calibration curves and linearity Calibration curves were constructed by plotting the concentrations of levocetirizine vs.
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