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At Happy Birthday, Alamo the Mosaic Man once again retuned to Astor Place, telling the story of how he began the infamous mosaic trail almost thirty years ago, while giving demonstrations of how he creates his mosaics. Across the entire year Astor Place, events have celebrated fifty years of the Cube, looking back at the significant cultural and social changes, which have taken place in our neighborhood.
At all of these events and on social media there has been one recurring question, what does the Cube mean to you? Several bacteria including P.
Michiels et al screened 31 bacterial genomes for CaBPs, and predicted that the P. We generated deletion mutants lacking efhP in P.
Since certain stress response proteins had reduced abundances in the proteomes of the efhP deletion strains, we assayed the mutants for resistance to chemical and oxidative stresses.
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Biofilm minimal medium BMM contained per liter: 9. Trace metal solution per liter of 0. Vitamins solution per liter: 0.
The pH of the medium was adjusted to 7. For experiments with no iron, trace metal solution contained no FeSO 4. For quantitative growth assays, cultures were pre-cultured in 5 ml tubes to mid-log phase, diluted to obtain optical density OD 600 of 0.
Growth data are based on at least 3 biological replicates.
Biofilm formation was quantified using abiotic solid surface assay in 96-well plates as described previously.
Prior to inoculation, overnight cultures were diluted to obtain equal optical densities. Nonattached cells were removed by washing the plates three times with saline solution 0.
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