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Paw pressure sensitivity for each animal is tested with an analgesymeter three hours after the carrageenan injection. See, Randall et al.
The effects of selected agents. This test see, Vinegar et al. The paw edema test is carried out using a plethysmometer for paw measurements.Except as required by law, we estimates and assumptions only as of forward-looking statements publicly, or to update.
At three hours post-carrageenan treatment, the volume of the treated paw left and the un-treated paw right is measured using a plethysmometer.
Von Frey Filament Test: The effect of a 3,4,6-substituted pyridazine on mechanical allodynia can be determined by the von Frey filament test in rats with a tight ligation of the L-5 spinal nerve: a model of painful peripheral neuropathy.
The surgical procedure is performed as described by Kim et al. A calibrated series of von Frey filaments are used to assess mechanical allodynia Chaplan et al.
Methods 19 -63. Filaments of increasing stiffness are applied perpendicular to the midplantar surface in the sciatic nerve distribution of the left hindpaw. The filaments are slowly depressed until bending occurred and are then held for 4-6 seconds.
The filament application order and number of trials were determined by the up-down method of Dixon Chaplan et al.